rnagents® promega kit - total rna isolation system (Promega)

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Promega rnagents® promega kit - total rna isolation system
Rnagents® Promega Kit Total Rna Isolation System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnagents%3A+total+rna+isolation+system/rna+isolation+kit+promega+rnagents/pm38614307-136-14-15
Average 90 stars, based on 1 article reviews

rnagents® promega kit - total rna isolation system - by Bioz Stars, 2026-09

90/100 stars

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Article Title: Acceleration of benzo(a)pyrene-induced colon carcinogenesis by Western diet in a rat model of colon cancer
Article Snippet: The standards were supplied to our laboratory by the NCI Chemical Carcinogen Repository within the Midwest Research Institute Repository located in Kansas City, MO. .. From all treatment groups total RNA was isolated using RNAgents Total RNA Isolation System (Promega, Madison, WI) and stored at −80 °C. cDNA was prepared from total RNA (2 μg) using oligo(dT)20 primers and AMV Reverse Transcriptase. ..

Article Title: Intestinal recruitment of CCR6-expressing Th17 cells by suppressing miR-681 alleviates endotoxemia-induced intestinal injury and reduces mortality.
Article Snippet: After 48 h, cells were harvested, washed with PBS, and subjected to a dual-luciferase reporter assay (Promega), according to the manufacturer’s instructions and using a Lumat LB 9507 luminometer (Berthold, Nashua, NH, USA). .. Total RNA was isolated from tissues and cells using the RNAgents Total RNA Isolation System (Promega), according to the manufacturer’s instructions. .. Subsequently, for miRNA quantification, with primer sets (RIBOBIO, Guangzhou, China), 40 ng of total RNA was reverse-transcribed into cDNA for quantitative PCR.

Article Title: Aerobic glycolysis enhances HBx-initiated hepatocellular carcinogenesis via NF-κBp65/HK2 signalling
Article Snippet: Reverse transcription was conducted using SuperScript IV Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA). qPCR was performed using a QuantiTect SYBR Green PCR Kit (Qiagen, Chatsworth, CA, USA). .. Total RNA was isolated from cells or liver tissues using the RNAgents Total RNA Isolation System (Promega, Madison, WI, USA) according to the manufacturer’s protocol. .. Reverse transcription was conducted using a Reverse Transcription Kit (TOYOBO, Japan) according to the manufacturer’s instructions. qPCR was performed using a Mini Opticon Real-time PCR System (Bio-Rad, Hercules, CA, USA) with SYBR Green (Invitrogen, Carlsbad, CA, USA). β-actin served as an internal control for qRT‒PCR.

Article Title: Evaluating genome-wide DNA methylation changes in mice by Methylation Specific Digital Karyotyping
Article Snippet: .. Total RNA was extracted from frozen lung tissue using the RNAgents total RNA isolation system (Promega, Madison, WI, USA). .. Equal amounts of total RNA (5 μg), as determined by the Ribo-Green RNA Quantification kit (Molecular Probes, Eugene, OR, USA), were used in a 20 μl cDNA synthesis reaction primed with oligo-dT (Superscript II; Invitrogen, Carlsbad, CA, USA).

Article Title: The expression profiles of chemokines, innate immune and apoptotic genes in tumors caused by Rous Sarcoma Virus (RSV-A) in chickens.
Article Snippet: Innate immune genes play an important role in the immune responses to Rous sarcoma virus (RSV)-induced tumor formation and metastasis.. Here, we determined in vivo expression of chemokines, innate immune and apoptotic genes in Synthetic Broiler Dam Line (SDL) chickens following RSV-A infection.. The mRNA expression of genes was determined at the primary site of infection and in different organs of progressor, regressor and non-responder chicks, using RT-qPCR.

Article Title: Stx2 Induces Differential Gene Expression and Disturbs Circadian Rhythm Genes in the Proximal Tubule
Article Snippet: .. From RPTEC cells, total RNA was extracted with RNAgents Total RNA Isolation System (Promega, Tokyo, Japan). .. Whole human genome oligonucleotide microarray (44K oligonucleotide DNA microarray, Agilent Technologies, Tokyo, Japan) was used for microarray experiments.

Article Title: Cooperative Interaction of Phenolic Acids and Flavonoids Contained in Activated Charcoal with Herb Extracts, Involving Cholesterol, Bile Acid, and FXR/PXR Activation in Broilers Fed with Mycotoxin-Containing Diets
Article Snippet: .. Total RNA was isolated using the RNAgents Total RNA Isolation System (Promega, Madison, WI, USA). .. RNA samples were reverse transcribed in a Programmable Thermal Controller (PTC)-100 (MJ Research, Watertown, MA, USA).

Article Title: Aerobic glycolysis enhances HBx-initiated hepatocellular carcinogenesis via NF-κBp65/HK2 signalling.
Article Snippet: Reverse transcription was conducted using SuperScript IV Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA). qPCR was performed using a QuantiTect SYBR Green PCR Kit (Qiagen, Chatsworth, CA, USA). .. Total RNA was isolated from cells or liver tissues using the RNAgents Total RNA Isolation System (Promega, Madison, WI, USA) according to the manufacturer’s protocol. .. Reverse transcription was conducted using a Reverse Transcription Kit (TOYOBO, Japan) according to the manufacturer’s instructions. qPCR was performed using a Mini Opticon Real-time PCR System (Bio-Rad, Hercules, CA, USA) with SYBR Green (Invitrogen, Carlsbad, CA, USA). β-actin served as an internal control for qRT‒ PCR.

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rnagents® promega kit - total rna isolation system (Promega)

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